Characterization of Sulfate Transport in Cultured Tobacco Cells
نویسندگان
چکیده
منابع مشابه
Characterization of sulfate transport in cultured tobacco cells.
Sulfate transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured in liquid medium was investigated.Transport was linear with time, had a sharp pH optimum between 6.5 and 7.5, and obeyed Michaelis-Menten kinetics. The Km varied within the range 2 x 10(-5)m and 4 x 10(-5)m and the maximum velocity was in the range 100 to 400 nanomoles per gram fresh weight.hour.Transport was inhibit...
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Sulfate transport by tobacco (Nicotiana tabacum L. var. Xanthi) cells cultured on either l-cysteine or sulfate as a sole sulfur source was measured. The transport rate on either sulfur source was low during pre-exponential growth, increased during exponential growth, and was maximal in late exponential cells. The initial increase in transport rate was correlated with a decline in the intracellu...
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Cysteine transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured on liquid B-5 medium was examined.Transport was linear with time or amount of tissue and had a pH optimum of 4.5. Cysteine transport over a wide concentration range was biphasic. The isotherm, for descriptive convenience, was divided into two segments both of which obeyed Michaelis-Menten kinetics. The Km for high a...
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Polar auxin transport is a crucial process for control and coordination of plant development. Studies of auxin transport through plant tissues and organs showed that auxin is transported by a combination of phloem flow and the active, carrier-mediated cell-to-cell transport. Since plant organs and even tissues are too complex for determination of the kinetics of carrier-mediated auxin uptake an...
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The sulfur requirements of tobacco (Nicotiana tabacum L. var. Xanthi) XD cells grown in chemically defined liquid media can be satisfied by sulfate, thiosulfate, l-cyst(e)ine, l-methionine or glutathione, and somewhat less effectively by d-cyst (e) ine, d-methionine or dl-homocyst (e)ine. Sulfate uptake is inhibited after a 2 hr lag by l-cyst (e)ine, l-methionine, l-homocyst(e)ine or l-isoleuci...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1976
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.58.3.358